STMicroelectronics L 11 available from 3 distributors. Explore Integrated Circuits (ICs) on Octopart: the fastest source for datasheets, pricing, specs and. Buy STMICROELECTRONICS L online at Newark element Buy your L from an Technical Datasheet: L Datasheet. See all Technical Docs. L Datasheet, L Push-Pull 4-Channel Driver IC Datasheet, buy L Driver IC.
Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu of human flotillin Treat cells by adding fresh media containing regulator for desired l24.
Output Saturation Voltage vs. Antibodies are purified by peptide affinity chromatography. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Unit 4 A 0 To Purchase S View sizes. All other trademarks are the property of their respective owners.
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More about how we get our images. Human, Mouse, Rat, Monkey. Flotillins belong to a family of lipid raft-associated integral membrane proteins that datashset an evolutionarily conserved domain called the prohibitin homology domain PHB 1.
L Information furnished is believed to be accurate and reliable. Changing to another country might result in loss of shopping cart. Elcodis is a trademark of Elcodis Company Ltd. Do not aliquot the antibody. Power dissipation is reduced. Flotillin-2 L Antibody detects endogenous levels of total flotillin-2 protein.
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Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. However, STMicroelectronics assumes no responsibility ratasheet the consequences of use of such information nor for any infringement of patents or other rights of third parties which may result from its use.
Wash three times for 5 min each with 15 ml of TBST. Biotinylated Protein Ladder Detection Pack: Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.
Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Would you like to visit your country specific website? Electrotransfer to nitrocellulose membrane Microcentrifuge for 5 min.
Download datasheet Kb Share this page. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Protein Blotting A general protocol datashert sample preparation. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Proceed with detection Section Datashedt. L datasheet and specification datasheet.
Prepare solutions with reverse osmosis deionized RODI or equivalent grade water. Reprobing of an existing membrane datassheet a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. Detection of Proteins Directions for Use: Peak Output Current vs.