• August 21, 2019

El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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When colonies developed into microcalli, the alginate beads were depolimerized. Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids. With their assistance, you can become well prepared and can avoid having to retake exams.

Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. The best study guides. This technique has created novel cellular genome configurations by combining sexually incongruent species. Plant Cell Rep 3: After that, protoplasts settled down.

To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis.

Cultivo de protoplastos de yuca y Stylosanthes

Somatoclonal protlplastos in potato: Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.


After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur. The same primer amplified four specific bands of, and bp in S.

Cultivo y fusion de protoplastos [1990]

After seven days, the culture medium was diluted protoplastox Protoplasts were precipitated by centrifugation at rpm during five minutes.

Earn while you study. Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome.

Plant Cell Rep This value is different from that obtained by Mattheij et al. The effect of initial culture media. Everything you need to know about selling on Stuvia. Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed.

A slot-blot hybridization method for screening somatic hybrids. Your fellow students know exactly where the pitfalls lie and what the key elements will be to your success in that module. The mixture was incubated for thirty minutes and then protoplasts were collected by centrifugation at rpm during five minutes.

Tema 9. Cultivo de protoplastos e hibridación somática

Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L. Plant Cell Tissue Organ Cult Prottoplastos characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers.


Protoplasts fusion from S. Root tips were stained by lacto-propionic orcein.

What do you want to do? Instpp Shoots also showed purple-colored meristematic zones. Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Somaclonal variation in potato. Tissue culture procedures to produce somatic hybrids protpplastos the application of molecular biological tools for their analysis are well established.

Non-transgenic applications of plant tissue culture in potato. Protoplasts were precipitated by centrifugation at rpm during 10 minutes. Introduction Potato, Solanum tuberosum L.


The other parent, the wild species S. In this paper we describe the fusion of leaf protoplasts of S. Isolation of plant DNA from fresh tissue.

Regeneration of isolated protoplasts to plants in Solanum dulcamara. Chromosome observations indicated that all hybrids were aneuploids. For the obtained somatic hybrid plants, the chromosome number protollastos from Table 2. Earn hundreds of dollars each month by selling your written material to your fellow students.